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Addgene inc
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Image Search Results
Journal: Nanomedicine : nanotechnology, biology, and medicine
Article Title: Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy
doi: 10.1016/j.nano.2020.102220
Figure Lengend Snippet: (A) Schematic diagram of NEP. (B) Delivery of Ascl1, Brn2 and Myt1l in MEF cells by NEP. Phenotypic characterization of induced neuron-like cells 2 weeks post-NEP (C) or 4 weeks post-NEP (D). Molecular markers are indicated on the top of each panel. Scale, 50 μM. (E) Ngf expression at weeks 1 and 4 post-NEP. NGF ELISA from differentiated MEF media at 4 weeks post-NEP (n = 10). RT-qPCR analysis of neurotrophin mRNA at (F) 1 week (n = 4) and (G) 4 weeks (n = 6) post-NEP. Data expressed as mean ± SEM, *P < 0.05.
Article Snippet: Ascl1 , Brn2 , and
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR
Journal: Nanomedicine : nanotechnology, biology, and medicine
Article Title: Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy
doi: 10.1016/j.nano.2020.102220
Figure Lengend Snippet: (A) Schematic diagram of TNT. (B) Confocal microscopic images showing three-plex in situ hybridization of Ascl1, Brn2, Myt1l, counterstained with DAPI. (C) RT–qPCR analysis of ABM gene expression in skin 24 h post-TNT. (n = 4), (D) Immunostaining showed TuJ1 fibers in skin. White dashed lines indicate epidermal and dermal junction. (E) Quantification of TuJ1+ fiber length per mm epidermis length. (n = 6) (F) Confocal microscopic images of skin showing co-localization (white) of FSP and TuJ1. (G) Quantification of TuJ1 and FSP positive cells per field of view. Data expressed as mean ± SEM (n = 3–4), *P < 0.05.
Article Snippet: Ascl1 , Brn2 , and
Techniques: In Situ Hybridization, Quantitative RT-PCR, Gene Expression, Immunostaining